Urinalysis | Urinalysis with Microscopy

UWHC Lab Test Details
Test NameUrinalysis with Microscopy
Test Code(s)UA
CPT Code(s)81001
MethodologyAutomated Urine Chemistry and Microscopy Analyzer
Days PerformedDaily, 24 hours.
In-Lab Turnaround Time4 hours.
Stat In-Lab Turnaround Time1 hour.
Collection Requirements
SpecimenUrine
Collection Container
Collection ContainerSterile screw cap container
Collection Instructions

First morning void preferred. Refrigerate if transportation to lab is delayed. Indicate whether specimen is midstream, indwelling catheter, single catheter or other method of collection. For midstream urine collection instructions refer to Health Facts for you - Male HF #4247, Female HF # 4236 located on UConnect.

Collection Volume10 mL
Pediatric Collection Volume3 mL
Specimen Processing Requirements
Stability
Stability Ambient

2 hours

Stability Refrigerated

24 hours

Stability Frozen

Not acceptable

Sample AnalyzedUrine aliquot
Testing Volume10 mL
Pediatric Testing Volume3 mL
Specimen Transport

Transport specimen to the laboratory immediately. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria

Samples held at room temperature for more than 2 hours or samples refrigerated for more than 24 hours are not acceptable.

Interpretation
Reference Interval

Age 0 days and up:

 

Dipstick:

 Specific Gravity *  1.005-1.035
 pH  5.0-8.0
 Protein  Negative
 Glucose  Negative
 Ketones  Negative
 Hemoglobin  Negative
 Bilirubin**  Negative     
 Leukocyte Esterase  Negative
 Nitrite  Negative
 Urobilinogen  0.2, 1.0, <2.0 mg/dL

 

Microscopic Findings:

WBC 0-5/hpf
RBC 0-2/hpf
Bacteria *** Negative

Squamous Epithelial cells ****

0-2/hpf

Renal Epithelial cells

Not seen or less than 1/hpf
Crystals

None Seen or Present calcium oxalate; Present amorphous urates or phosphates

Casts 0-4 hyaline casts/lpf
Effective Date of Reference RangeWed, 09 Jan 2013 14:47:00 CST
Critical CallsView here
Additional Information

Conversion and Sensitivity Chart Link

 

* All UW Health Laboratories may use a screening method for specific gravity, based on a colorimetric test strips which reports only the ionic solutes. If a specific gravity is performed via colorimetric test strip, the following            comment will appear: Only ionic solutes measured. For both ionic and nonionic solutes order “Specific Gravity, Urine”.The refractometer method is routinely included in urinalysis performed at the UH and TAC Laboratories and is the method used when "SPECIFIC GRAVITY, URINE" is ordered. The refractometer method includes both ionic and non-ionic solutes. Included in ionic solutes are sodium, chloride, sulfate, phosphate, and ammonium. Included in non-ionic solutes are urea, glucose, and protein. The reportable range is 1.000 to ≥1.050.

** Positive screen not confirmed. Clinical correlation recommended.

*** If bacteria are undetected or present at very low levels, bacteria will be reported as NEGATIVE. Higher levels will be reported as PRESENT or MANY.

        The presence of bacteria is not necessarily indicative of urinary tract infections. (Van Nostrand, J.D. et al, Am J Clin Path 113:709-713).

**** Squamous Epithelial Cells (SEC), present in a urine specimen likely enter from sites distal to the bladder. SEC may indicate the possibility of other contaminants such as bacteria. Clean catch (midstream) urine collection may reduce the possibility of contamination. For midstream urine collection instructions refer to Health Facts for you:

Male: https://uconnect.wisc.edu/clinical/pted/hffy/genitourinary/4247.hffy

Or Female: https://uconnect.wisc.edu/clinical/pted/hffy/diagnostic-tests/4236.hffy

A comment will indicate high levels of SEC. Results of all analytes will be reported, and the provider should decide if recollection is needed.