Immunology | SSB Ab

UWHC Lab Test Details
Test NameSSB Ab
Test Code(s)HCSSB, SSB
CPT Code(s)86235
MethodologyEnzyme Immunoassay
Clinical Information

Antibodies to extractable nuclear antigens (SSA, SSB, RNP, Sm) are found in many connective tissue diseases including SLE (SSA, SSB, RNP, SM), mixed connective tissue disease (high titer RNP), Sjogren's syndrome (SSA, SSB) and scleroderma (SSA, RNP). Sm is very specific for SLE, but not sensitive (30%). Mixed connective tissue disease is almost always (90%) associated with high titer RNP and is associated with antibodies to the 70kd protein by Western blot. SSA is found in approximately 70% of patients with Sjogren's syndrome, whereas SSB is found in 30% of patients. Scleroderma is typically associated with centromere or Scl-70 antibodies, but RNP and SSA are found in a small proportion of patients.

Testing SiteUniversity Hospital
Days PerformedMon-Fri, dayshift - day varies.
In-Lab Turnaround Time1 week.
Collection Requirements
Collection Container
Collection ContainerGold top (SST)
Also AcceptableRed cap with yellow ring (SST), Red top
Collection Volume3 mL
Pediatric Collection Volume1 mL
Specimen Processing Requirements
Stability Ambient

8 hours

Stability Refrigerated

7 days

Stability Frozen

No limit at-20°C or lower. Avoid repeat freeze and thaw.

Sample AnalyzedSerum
Testing Volume1 mL
Pediatric Testing Volume0.3 mL
Specimen Processing

Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.

Outreach Specimen Processing

Centrifuge. If a complete barrier has not formed, transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport

Transport specimen to laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria

Hemolyzed, grossly lipemic/turbid and icteric specimens are not acceptable.

Reference Interval

Normal SSB (0 days and up): < 20

SSB Interpretive Guidelines
Negative < 20
Weak Positive 20-39
Moderate Positive 40-80
Strong Positive >80