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UW Health SMPH
American Family Children's Hospital

The Small Molecule Screening and Synthesis Facility (SMSSF) fosters collaborative drug discovery research between chemists, biologists and physician scientists that leads to the identification of new small molecules that inhibit disease targets and phenotypes in biochemical and cellular models relevant to any therapeutic area.


Our drug discovery services include:

News and Accomplishments


Tip-less, acoustic liquid dispensing of aqueous and DMSO solutions in 2.5nanoliter(!) and up volumes into 96, 384 and 1536-well plates is now available for any application on an Echo 555.

SMSSF is the home of a new Seahorse Bioscience XFe96 Extracellular Flux Analyzer which simultaneously interrogates mitochondrial respiration and glycolysis - in a microplate, in real-time. The XFe96 Analyzer determines in vitro oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in order to assess cellular functions such as oxidative phosphorylation, glycolysis and fatty acid oxidation. 

The chemists at SMSSF have designed, synthesized and purified over 60 new analogs for one project that have been tested in biochemical and cell-based assays to select two compounds
that are now being evaluated for anti-cancer activity in mouse models.

With pilot project funding from Institute for Clinical and Translational Research (ICTR) and use of the UW Center for High-throughput computing we have expanded our virtual screening capabilities to two million compounds on one binding pocket in one run and queries of over 100 protein binding pockets for compound binding.


View the Small Molecule Screening and Synthesis Facility's Scientific Accomplishments


Acknowledging SMSSF


Funding received from the UWCCC Cancer Center Support Grant (CCSG) for published research, including the use of UWCCC Shared Resources to generate or analyze data, or conduct clinical trials, needs to reference the UWCCC. See Acknowledging UWCCC in Publications, Posters and Presentations


View the UW Drug Discovery and Development website for more information on other UW-Madison laboratory facilities that provide state-of-the-art instrumentation and expert scientific staff.




Faculty Leader
Michael Hoffmann 
(608) 265-8687 

Facility Managers
John Feltenberger 
(608) 263-2644

HTS and Profiling Assays
Gene Ananiev  
(608) 265-8687

Computational Modeling and Virtual Screening 
Ken Satyshur 

Wisconsin Institutes for Medical Research 
Room 6003 
1111 Highland Avenue
Madison, WI 53705

Advisory Board