American Family Children's Hospital

Mycoplasma Detection

Mycoplasma contamination is one of the most common and serious problems in culturing eukaryotic cells because it alters the phenotypic characteristics of the cells and can negatively impact results. Since mycoplasma is typically not visible and does not respond to antibiotics, an alternative sensitive and reliable detection method is required. Small Molecule Screening Facility (SMSF) uses the R&D Systems Mycoprobe Mycoplasma Detection Kit. This assay is capable of detecting the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination: M. hyorhinis, M. arginini, M. fermentans, M. orale, M. pirum, M. hominis, M. salivarium and Acholeplasma laidlawii.
The SMSF staff scientists test each client-provided sample in triplicate along side positive and negative controls.

Assays are usually run the week that samples are submitted and data are available within 48 hours of assay completion.