American Family Children's Hospital

Mechanism of Action Assays

The Small Molecule Screening Facility (SMSF) offers a broad range of biological profiling assays to help identify a chemical compound's biological activity and potency including:

  • AlphaLisa and AlphaScreen Assays for Biomarkers, Cytokines, Phospho-proteins and more
  • AKT Phosphorylation
  • Anti-microbial Assays
  • Apoptosis Assays
  • Cell Migration
  • Cytotoxicity and Cell Proliferation Assays
  • DNA Damage Assays
  • Enzyme Activity
  • ERK1/2 Phosphorylation
  • HDAC 
  • NFKb
  • Phosphatase Assays (PP1A and PP2A)
  • Phosphorylation
  • Protein-DNA Binding
  • Protein-Protein Binding
  • Reporter Gene Expression
  • Signal Transduction
  • TGF-beta signaling
  • Tubulin Polymerization
  • Viral Entry
  • WNT -Beta Catenin (Super Topflash Assay)
  • Wound Healing and Chamber Migration Assays

Custom assay development services are also available.


Contact us for more information.