Richard Halberg, PhD – Chemoprevention Program
Mice expressing an activated form of PI3K kinase develop intestinal obstruction due to large proximal colontumors. The tumors exhibit a high avidity for FDG (A) and NM404 (D). Mice were injected with an imaging agent, scanned, and the data was reconstructed using standard algorithms. Following scan acquisition, each mouse was sacrificed and the abdominal wall was dissected revealing tumors in the colon (B and E, arrows). The intestine was removed, split lengthwise, and splayed out to expose the luminal surface. Areas of hyperplasia in the small bowel (C, red box) and tumors in the colon (C and F, arrows) were evident. Scale bars: 1 cm.
Leystra AA, Deming DA, Zahm CD, Farhoud M, Paul Olson TJ, Hadac JN, Nettekoven LA, Albrecht DM, Clipson L, Sullivan R, Washington MK, Torrealba JR, Weichert JP, Halberg RB. Mice Expressing Activated PI3K Develop Advanced Colon Cancer. Cancer Res. 2012, in press. Grant acknowledgment: American Society of Clinical Oncology Young Investigator Award Development of a Mouse Model for the Screening of Targeted Pharmacologic Agents.
Ajit Verma, PhD – Chemoprevention Program
Transgenic TRAMP (TG//WT) and PKCε knockout TRAMP (TG//KO) male mice wereevaluated for the development of PCa. Hybrid microPET/CT image acquired 48h post 124I-NM404IV injection to TG//WT (A), TG//KO (B) mice at 18 weeks. Whole body (Ai, Bi) and excised tissue (Aii, Bii) are shown. U, LN, P denote urinary bladder proximal lymph node and PCa respectively. Arrows indicate metastases of PCa in the lung, kidney, and lymph node of TG//WT mice.
Hafeez BB, Zhong W, Weichert J, Dreckschmidt NE, Jamal MS, Verma AK. Genetic ablation of PKC epsilon inhibits prostate cancer development and metastasis in transgenic mouse model of prostate adenocarcinoma. Cancer Res. 2011;71(6):2318-27. PMCID: PMC3059775. Grant acknowledgment: 5R01CA102431-07 TNF Alpha in PKC Epsilon Signaling to Carcinogenesis.
Sean Fain, PhD – Imaging and Radiation Science Program
In vivo T1 monitoring using a gadolinium-based contrast agent. (a) Gd –chelate concentration and pyruvate signal intensity over time in the liver of a rat (b-outline). (b) An image of 13C-pyruvate is shown overlaid on the 1H image for the same experiment prior to the contrast agent injection (t = 8 s). (c) An image from a later time point of the same animal (t = 16 s). Note that the vascular signal from 13C pyruvate is substantially reduced, while the 13C-pyruvate signal in the tissue remains.
Smith MR, Peterson ET, Gordon JW, Niles DJ, Rowland IJ, Kurpad KN, Fain SB. In vivo imaging and spectroscopy of dynamic metabolism using simultaneous 13C and 1H MRI. IEEE Trans Biomed Eng, 2012;59(1):45-9. Grant acknowledgment: T32 CA009206-28.