The UW Carbone Cancer Center (UWCCC) Proteomics Shared Resource is incorporated in the University of Wisconsin Biotechnology Center (UWBC) Mass Spectrometry facility.
The goal is to provide UWCCC researchers access to state of the art mass spectrometry and gel electrophoresis instrumentation for proteomic and metabolomics research. In addition they are able to access tools and expertise to help with experimental design and data interpretation.
Current instruments include a ThermoFisher Scientific Orbitrap XL with ETD, an AB Sciex 5500 QTRAP, an AB Sciex 4800 MALDI TOF/TOF, an Agilent LC/MSD TOF, a ThermoFisher TSQ Quantum Discovery Max and an AB Sciex 3200 QTRAP. These instruments span a range of mass spectrometric technologies and capabilities. With these instruments, the Proteomics Shared Resource has the ability to identify and quantify diverse molecular species, including proteins, peptides, oligonucleotides and small molecules, as well as conduct proteomic and phosphoproteomic profiling and quantitation and metabolite profiling and quantitation.
In addition to measuring the molecular weight of compounds, the instruments are designed to perform MS/MS so that the structure of a selected ion can be deduced from the fragments. This is particularly useful for peptide sequencing and post-translational modification mapping. The four ESI-MS are equipped with HPLC systems for LC/MS/MS experiments. LC/MS/MS is extremely useful for quantitation of known compounds and for the rapid screening of a mixture to identify the components of interest. Capability exists to perform proteolytic digestions of proteins, which can be further analyzed by HPLC, MS, LC/MS and MS/MS for peptide mapping and peptide sequencing.
The Mass Spectrometry/Proteomics/Metabolomics facility is utilized in many ways to support cancer related research. These range from routine measurements of synthetic compounds to specialized projects targeting biomarkers. The facility can adapt to address new projects as they are conceived.
Learn more about the Mass Spectrometry / Proteomics facility at the UW Biotechnology Center