Shared Resources Equipment
Please review Flow Cytometry - Using the Facilitybefore making an appointment. Clicking on any of the equipment below will link you to the online scheduling system to make an appointment. The system sends email reminders automatically. Please do not email the scheduler because the staff does not respond to that email. Direct all queries to staff email addresses.
You MUST attend the Introduction to Flow Cytometry lecture series before signing up for any training on any cytometer. (Training on microscope is excluded.) Once you have attended the lecture series make an appointment for two hours on:
04. FACSCalibur-WIMR-Training/Assisted (Tuesday 12-3pm)
08. LSRII-WIMR-Training/Assisted (Wednesday 12-3pm)
10. MACSQuant-WIMR-Training/Assisted (Thursdays, 12-3pm)
12. BD Pathway-WIMR-Training/Assisted (Mondays, 12-3pm)
The two-hour hands-on training is intended for those with little or no flow cytometry experience. It will teach the basics of instrument set-up and data collection. Fluorescent Beads will be used for training. Please do not bring cell samples.
Once the two-hour training is completed or for those who have some experience operating a flow cytometer, it will be necessary to schedule an hour of assisted time with user samples to discuss specific assay data collection. No user will be allowed to operate the cytometers independently until these training sessions are completed.
Analysis Computers
Offline data analysis computers are available to laboratory users only and are located at both the 7th floor WIMR location and at the McArdle laboratory. Computers are loaded with a variety of analysis packages including FlowJo, CellQuestPro, FCS Express, ModFit, WinList, Image analysis, as well as standard Office software.
There is no charge for the use of these computers. However, they must be used exclusively for the analysis of data generated at this laboratory.
You may reserve a computer online, but they are also available on a first-come, first-served basis.
Make an appointment for:
02. Analysis Computers - 7016 WIMR
PComputers available for data analysis. Two Macs (running FlowJo and ModFit). One Mac (running CellQuest). One PC (running Attovision, FlowJo, ModFit). One PC (Running BD FCap Array, ModFit).
03. Analysis Computers - McArdle
Computers with FlowJo, ModFit, WinList and other analysis software. Two Macs. One PC.
Benchtop Analyzers
The benchtop instruments used for this purpose are the FACSCalibur (488nmand 633nm lasers) or the LSRII (488nm, 405nm, UV, and 633nm lasers).
When signing up for appointments here, insure all fluorochromes to be used can be excited with the lasers listed. The instruments are available for self-run appointments for which no assistance is provided, or for staff-assisted appointments for those who need assistance in data collection or instrument operation. Staff-assisted appointments can be used by those who have not received any instrument training but need samples analyzed, as well as by those who need assay-specific instruction.
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Instrument Maps:
Make an appointment for: 04. FACSCalibur-WIMR-Training/Assisted 05. FACSCalibur-7016 WIMR, Self schedule All users operating cytometers after staff hours (8pm-6am) are responsible for shutting down the cytometer after use. Please contact staff with any questions.
06. FACSCalibur-McArdle, Self schedule
09. LSRII-7016 WIMR, Self schedule
10. MACSQuant Training/Assisted New User Training: Schedule two hours. Assisted appointments: For those who need assistance operating benchtop instuments for data collection.
11. MACSQuant Self
The facility has two high-speed sorters: one located in the McArdle laboratory and one at the 7th floor WIMR location. Each sorter has some unique capabilities for the maximum applications, but they both can sort up to four separate populations simultaneously. Operated by staff ONLY. They are both housed under BSL2 conditions. Note: Samples exceeding BSL2 levels when aerosolized cannot be sorted.
Sorter Maps:
Make an appointment for: The SORP BD FACSAria is a digital system equipped with five lasers (UV, 405 nm, 488 nm, 532 nm and 640 nm). The Aria can simultaneously collect fluorescent emissions from 16 different fluorophores. Please check with us if you have questions regarding fluorophore compatibility.
The FACS AriaIII is a cell sorter equipped with four lasers (403 nm, 488 nm, 561 nm and 637 nm). This sorter is contained in a custom Biological Safety Cabinet. Sorts containing human cells, retroviral transfections or as determined in your lab's Biological Safety Protocol MUST be sorted on this instrument.
Microscopes
Make an appointment for: 14. BD Pathway-7016 WIMR, Assisted
The Pathway is a microscope with a temperature-controlled chamber and liquid handling capabilities. This inverted fluorescent scope requires slides or 96/384 well microtiter dishes. It is equipped with two mercury arc lamps, 16 different excitation filter sets and eight different emission filter sets. It uses standard immunofluorescent probes, like Texas Red, Hoechst, and the Alexa dyes and can collect either regular or confocal images. 16. Fluorescent Microscope-7016 WIMR schedule The Olympus BX51 Fluorescent Microscope is capable of brightfield, darkfield and fluorescent microscopy. Attached to a CCD camera and PC for taking pictures. Self-run appointments only.
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