American Family Children's Hospital

Pharmacodynamic Assays

The Cancer Pharmacology Core will validate and run a variety of PD assays, either as a single assay or multiplexed with the Bioplex.


The following assays have been validated: 

  • Bone health panel: Parathyroid Hormone, Retinol, Calcium
  • Histone acytylation via chromatin precipitation (p21, HSP70, Nur77, CYP3A4)
  • HIF-1a
  • Prostate cancer panel: IGF1, IGFBP3, Testosterone, PSA, Urine 2-Hydroxyestrogen and 16alpha-hydroxyestrone
  • Reactive oxygen species via electron paramagnetic resonance imaging
  • TGF1, TGF2
  • VEGF pathway (VEGF, bFGF, MIP1, VEGFR1, VEGFR2) 

Please email Jill Kolesar to request any of these analytical services.