Pharmacogenetic Assays

Gene Expression Assays

Gene expression assays are performed by real time PCR with a reaction specific internal standard. The 3P Lab provides comprehensive services, including development of primers and internal controls, RNA extraction from cell culture, peripheral blood mononuclear cells, paraffin embedded tissue, and other matrices, assay performance and data analysis.

Genotyping and Mutation Assays

The 3P Lab provides comprehensive genotyping and mutation assay services, including assay development, performance and data analysis. Genotyping is typically performed in peripheral blood mononuclear cells by pyrosequencing, while mutation analysis is performed in tumor samples after laser capture microdissection (LCM) or manual microdissection. CLIA-level genotyping and mutation analysis available.

New for 2012: The Ion-Torrent PGM™ system

• AmpliSeq: able to identify mutations in approximately 30 cancer genes with a starting template of 10ng of DNA.
• RNA-Seq analysis
• Targeted gene sequencing

Genotyping Assays

• Drug Transporter Genotyping
  ABCB1, ABCG2
  CYP3A4

• Drug Metabolism Genotyping
  CYP1A1,CYP1A2, CYP2B6, CYP2C9, CYP3A4/5, CYP17A, CYP2C8, CYP2C19, CYP2D6
  NQO1
  UGT1A1, UGT1A4, UGT, 1A9
   
• Drug Target Genotyping
  EGFR TA repeat
  VEGFR1 VEGFR2 VEGFR
  ERCC1

Cancer Mutation Analysis

• AmpliSeq panel
• c-MET
• EGFR
• IgVh receptor
• Kras