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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: FISH - SRY, Yp11.3

Test Code(s): 871F34 / HC871F34

CPT Code(s): 88271, 88273

Clinical Significance: Fluorescence in situ hybridization performed on metaphase cells using DNA probes (LSI SRY; Vysis, Inc) specific for Sex-determining Region Y (SRY), simultaneously with a probe for the centromere of chromosome X (CEP X) as a control.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Sat.

Turnaround Time: Routine: 10 - 14 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: This blood must be received and processed by WSLH within 24 hours of collection.  Blood must be received by the UWHC Core Laboratory Monday-Friday.

Collection Container: Preferred: Green top (with sodium heparin anticoagulant)

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Specimen Transport: Transport specimen to laboratory. Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Blood that is collected in EDTA, sodium citrate, or other anti-coagulants is not acceptable. Blood that is clotted or hemolyzed is not acceptable. Blood must not be frozen. Plasma and serum are not acceptable.


Interpretation

Report provides interpretation.

Test Limitations:

Very small deletions or point mutations are not detected by this assay.

Sex determining region on Y

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