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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Pain Management Profile, Urine

Test Code(s): UPAIN / HCUPAIN

CPT Code(s): 80304

Methodology: Liquid Chromatography-Tandem Mass Spectrometry

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Routine: 2 - 3 days.

Specimen Requirements

Specimen: Random urine

Collection Container: Preferred: Sterile screw cap container

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Urine aliquot

Volume Required: Preferred: 3 mL
Pediatrics: 1 mL

Stability: Ambient: 3 days
Refrigerated: 2 weeks
Frozen: 1 year



The following is a list of cut-off ranges that are 

used to determine the level (Low, Moderate, or

High) of each of the 35 drug substances assayed in the Pain Management Profile.    

TEST: 6-MAM,amphetamine, benzoylecgonine (cocaine metabolite), MDA, MDMA, methamphetamine, codeine, hydrocodone, hydromorphone, meperidine, normeperidine, methadone, EDDP, morphine, naltrexone, oxycodone, oxymorphone, tapentadol, N-desmethyltapentadol, and tramadol
  Below Low Moderate High
   (ng/mL)  (ng/mL)  (ng/mL)  (ng/mL)
   <25  25-300  301-1000 >1000 
TEST: oxazepam, buprenorphine, norbuprenorphine, and naloxone
  Below   Low Moderate   High
  (ng/mL)   (ng/mL)  (ng/mL) (ng/mL) 
   <10 10-300 301-1000  >1000
TEST: fentanyl, norfentanyl   
  Below   Low Moderate   High
  (ng/mL)   (ng/mL)  (ng/mL) (ng/mL) 
   <7  7-50  51-200  >200
TEST: alprazolam, hydroxyalprazolam, 7-aminoclonazepam, diazepam, nordiazepam, lorazepam, midazolam, temazepam
  Below   Low Moderate   High
  (ng/mL)   (ng/mL)  (ng/mL) (ng/mL) 
   <5 5-300 301-1000  >1000
TEST: N-desmethyltramadol
   Below  Low Moderate   High
  (ng/mL)  (ng/mL)  (ng/mL)  (ng/mL) 
  <100 100-300 301-1000 >1000



Additional Information:

The Pain Management Profile simultaneously screens and confirms the presence of 35 drug substances in human urine by LC-MS/MS and reports these drugs in semi-quantitative ranges (Low, Moderate, and High).  The assay is for the 35 drug substances listed below. No other drug substances will be detected or reported.


DRUGS OF ABUSE CLASS: 6-MAM (Heroin metabolite), amphetamine, benzoylecgonine (cocaine metabolite), MDA, MDMA, and methamphetamine.

BENZODIAZEPINES CLASS: alprazolam, hydroxyalprazolam, 7-aminoclonazepam (clonazepam metabolite), diazepam, nordiazepam, lorazepam, midazolam, oxazepam, and temazepam.

OPIOIDS CLASS: buprenorphine, norbuprenorphine, codeine, fentanyl, norfentanyl (fentanyl metabolite), hydrocodone, hydromorphone, meperidine, normeperidine (meperidine metabolite), methadone, EDDP (methadone metabolite), morphine, naloxone, naltrexone, oxycodone, oxymorphone, tapentadol, N-desmethyltapentadol (tapentadol metabolite), tramadol, N-desmethyltramadol (tramadol metabolite).


Pain Management Profile Quick Reference and Interpretation Guide (pdf)


NOTE: Please call (608) 263-7029 with any questions. 


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories.  This test meets the standards for clinical testing.  This test should not be regarded as investigational or for research use.  The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.


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