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UW Health SMPH

UW Hospital and Clinics Lab Test Directory

Test Name: HLA Class I and II Ab Screen

Test Code(s): HSCB / HCHSCB (if Pre-Tx), HSCS / HCHSCS (if Post-Tx or Bone Marrow Recipient)

CPT Code(s): 86807

Methodology: Luminex xMAP (Multi-Analyte Profiling)

Clinical Significance: Test used for the detection and identification of antibodies to HLA class I (A-, B-, C-loci) antigens by single-antigen bead-based flow cytometry.

Days Performed: Three times per week.

Turnaround Time: Stat: Not available stat.
Routine: 2 - 3 working days.

Specimen Requirements

Specimen: Blood

Patient Preparation: If patient is on dialysis, draw specimen pre-anticoagulation.

Collection Instructions: Test may be analyzed on serum specimens already in the UWHC HLA Laboratory. Indicate on order to use most recent sample in lab.

Collection Container: Preferred: Red top

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.1 mL

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) immediately.  Separate serum from clot if potential for hemolysis exists.  Transport at room temperature or on coolant pack if coming from outreach location.

Unacceptable Criteria: Specimens greater than 3 days old and grossly hemolyzed samples are not acceptable.

Stability: Ambient: 3 days
Refrigerated: 7 days
Frozen: 14 days (serum)


Results will list calculated PRA (percent reactive antibody) of class I (HLA-A, -B, or -C locus) antibodies detected, and if HLA specificities are identified, will be reported in a separate scanned report.

Additional Information:

This test utilizes flow cytometry-based detection of HLA class I antibodies by HLA single antigen-bound beads (Luminex). This test has high sensitivity and high specificity and can identify antibody specificities in highly-sensitized patients. Target beads are bound with individual HLA-A, HLA-B, or HLA-C locus antigens on each bead. Panel Reactive Antibody (PRA) result is expressed as a calculated PRA based on UWHC donor population HLA frequencies.


This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.

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