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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: AF4/MLL Fusion Transcript, t(4;11)(q21;q23), PCR

Test Code(s): 887M30 / HC887M30

CPT Code(s): 81401

Clinical Significance: To detect AF4/MLL fusion transcripts associated with ALL. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Fri.

Turnaround Time: Routine: 3 - 5 days.


Specimen Requirements

Specimen: Blood or bone marrow

Collection Instructions: Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 2-3 mL blood or 0.5-1.0 mL bone marrow

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) at room temperature (may transport with coolant during hot, >85 weather).

Unacceptable Criteria: Whole blood or bone marrow that is clotted or frozen.  Plasma and serum are not acceptable.

Stability: Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Interpretation provided with results.

Test Limitations:

Failure to identify an abnormal clone by RT-PCR does not rule out the presence of other abnormalities in the specimen.

t(4;11)(q21;q23)

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