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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: EBV Ab to Early Ag, IgG

Test Code(s): EBVEA / HCEBVEA

CPT Code(s): 86663

Methodology: ELISA

Clinical Significance: The intent of the test system together with other clinical information is to aid in the determination of immune status, and/or aid in the diagnosis of Epstein-Barr Virus (EBV).

Days Performed: Once a week.

Turnaround Time: Stat: Not available stat.
Routine: Up to 4 days

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge.  Transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to the laboratory. Transport with coolant pack if coming from satellite or outreach location.

Unacceptable Criteria: Grossly hemolyzed/ lipemic, heat-treated serum, microbially contaminated and serum containing heavily-visible particulate.

Stability: Ambient: 8 hours
Refrigerated: 7 days
Frozen: 3 months at 20C or greater


Negative: No detectable IgG antibodies to EBV-EA. 
Equivocal: Immune status cannot be determined.
Positive: Reactive for IgG antibodies to EBV-EA.


All equivocal test results will be repeated within two working days.

Test Limitations:

This test detects both R and D components of EBV Early Antigen. The test can not differentiate between antibodies to the R and D components of EA.


The magnitude of the measured result above the cutoff is not indicative of the total amount of antibody present and cannot be correlated to IFA titers.

Epstein Barr virus, Epstein-Barr virus,EBV-EA IGG, Early Ag, EBV EA

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