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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: HLA High Resolution, DR Locus

Test Code(s): HRDRB1LOC / HCDRBSEQ

CPT Code(s): Multiple, varies by locus

Test Description: Test Component:

Orderable by locus, but typically for stem cell transplant all HLA loci would be ordered on the recipient and donor: A-locus, B-locus, C-locus, and DRB1- locus.


Methodology: PCR followed by Direct Sequencing

Clinical Significance: High-resolution identification of HLA alleles, typically used for stem cell donor/recipient matching.

Days Performed: Mon-Fri, dayshift - day varies.

Turnaround Time: Stat: Not available stat.
Routine: 7 - 10 days.


Specimen Requirements

Specimen: Blood

Patient Preparation: No transfusions (RBC, WBC, Platelets) within 24 hours prior to collection.

Collection Container: Preferred: Lavender top
Also Acceptable: Yellow top(with ACD-A/B anticoagulant)

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220).

Stability: Ambient: 7 days
Refrigerated: 3 weeks
Frozen: Not acceptable


Interpretation

A written interpretive report will be provided.

Test Limitations:

Identification of HLA alleles generally at the single allele level. Certain combinations may not be resolvable to allele-level identification.

Additional Information:

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.

sequencing

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