UW Hospital and Clinics Lab Test Directory
| Test Name: |
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EBV Ab to Nuclear Ag, IgG
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| Test Code(s): |
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EBVNA / HCEBVNA
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| CPT Code(s): |
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86664
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| Methodology: |
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ELISA
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| Clinical Significance: |
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The intent of the test system together with other clinical information is to aid in the determination of immune status, and/or aid in the diagnosis of EBV (Epstein-Barr Virus)
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| Days Performed: |
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Once a week.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: Up to 4 days
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Specimen Requirements
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| Specimen: |
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Blood
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| Patient Preparation: |
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None
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| Collection Instructions: |
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None
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| Collection Container: |
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Preferred: Red cap with yellow ring (SST)
Also Acceptable:
Red top
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| Collection Volume: |
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Preferred: 1 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 0.5 mL
Pediatrics: 0.3 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to the laboratory. Transport with coolant pack if coming from satellite or outreach location.
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| Unacceptable Criteria: |
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Grossly hemolyzed/ lipemic, heat-treated serum, microbially contaminated and serum containing heavily-visible particulate.
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| Stability: |
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Ambient: 8 hours
Refrigerated: 7 days
Frozen: 3 months at 20°C or greater
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Interpretation
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| Negative: |
No detectable IgG antibodies to EBNA-1. Patient is presumed to have no current or previous infecton with EBV and is susceptible to a primary infection. |
| Equivocal: |
Immune status cannot be determined. |
| Positive: |
Reactive for IgG antibodies to EBNA-1 indicating a past EBV infection. |
All equivocal test results will be repeated within two working days.
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| Test Limitations: |
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Patients with severe immunocompomised conditions may show negative results for EBNA antibodies even if antibodies to VCA are present. Likewise, an anti-EBNA response may fail to develop in patients who have an immunodeficiency disease or who are immunosuppressed.
This assay detects antibody to the EBNA-1 antigen and no other EBNA antigens.
The numeric value (OD Ratio) obtained for a reactive specimen is not indicative of the amount of antibody present and cannot be correlated to an antibody titer.
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Epstein-Barr Virus,Epstein Barr Virus, EBV,EBV NA Immune Status,EBVNA,EBNA,EBNA-1,EBV nuclear antigen-1 IgG,EBV nuclear antigen-1 antibodies
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