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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: EBV Ab to Nuclear Ag, IgG

Test Code(s): EBVNA / HCEBVNA

CPT Code(s): 86664

Methodology: ELISA

Clinical Significance: The intent of the test system together with other clinical information is to aid in the determination of immune status, and/or aid in the diagnosis of EBV (Epstein-Barr Virus)

Days Performed: Once a week.

Turnaround Time: Stat: Not available stat.
Routine: Up to 4 days

Specimen Requirements

Specimen: Blood

Patient Preparation: None

Collection Instructions: None

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge.  Transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to the laboratory. Transport with coolant pack if coming from satellite or outreach location.

Unacceptable Criteria: Grossly hemolyzed/ lipemic, heat-treated serum, microbially contaminated and serum containing heavily-visible particulate.

Stability: Ambient: 8 hours
Refrigerated: 7 days
Frozen: 3 months at 20C or greater


Negative: No detectable IgG antibodies to EBNA-1.  Patient is presumed to have no current or previous infecton with EBV and is susceptible to a primary infection.
Equivocal: Immune status cannot be determined.
Positive: Reactive for IgG antibodies to EBNA-1 indicating a past EBV infection. 


All equivocal test results will be repeated within two working days.

Test Limitations:

Patients with severe immunocompomised conditions may show negative results for EBNA antibodies even if antibodies to VCA are present. Likewise, an anti-EBNA response may fail to develop in patients who have an immunodeficiency disease or who are immunosuppressed.


This assay detects antibody to the EBNA-1 antigen and no other EBNA antigens.


The numeric value (OD Ratio) obtained for a reactive specimen is not indicative of the amount of antibody present and cannot be correlated to an antibody titer.

Epstein-Barr Virus,Epstein Barr Virus, EBV,EBV NA Immune Status,EBVNA,EBNA,EBNA-1,EBV nuclear antigen-1 IgG,EBV nuclear antigen-1 antibodies

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