| Test Name: |
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Flow Cytometry, CD19 and CD20
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| Test Code(s): |
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CD1920 / HCCD1920
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| CPT Code(s): |
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86355, 86356, 88185
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| Test Description: |
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Test Components: B-Cell Antigens- CD19,CD20, Other Antigens- CD45
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| Days Performed: |
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Mon-Fri, dayshift- specimens accepted at anytime.
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| Turnaround Time: |
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Routine: Approximately 2 working days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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Outside clients should send WBC and Differential results with specimen. If no results accompany request, a WBC and Differential will be added (see transport and limitations for more information)
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| Collection Container: |
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Preferred: Lavender top
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 2.5 mL
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| Sample Analyzed: |
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Whole Blood
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| Volume Required: |
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Preferred: 2 mL
Pediatrics: 1 - 2 mL
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| Specimen Processing: |
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Keep specimen at room temperature. Do NOT centrifuge tube.
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| Specimen Transport: |
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Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. Maintain ambient temperature during transport. For outreach locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.
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| Unacceptable Criteria: |
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Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Specimens older than 24 hours at the time of analysis are not acceptable.
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| Stability: |
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Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Reference Interval:
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Age 18 years and up
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absolute number/uL |
| CD3-CD19+ |
6-24 |
77-427 |
| CD20 |
5-16 |
60-280 |
Please contact UWHC Flow Cytometry Laboratory at (608) 263-3868 for pediatric reference intervals.
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| Additional Information: |
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This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
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