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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, CD19 and CD20

Test Code(s): CD1920 / HCCD1920

CPT Code(s): 86355, 86356

Test Description: Test Components: B-Cell Antigens- CD19,CD20

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

Turnaround Time: Routine: 1 - 4 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: Outside clients should send WBC and Differential results with specimen. If no results accompany request, a WBC and Differential will be added (see transport and limitations for more information)

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 2.5 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 2 mL
Pediatrics: 1 - 2 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. Maintain ambient temperature during transport. For outreach locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Specimens older than 24 hours at the time of analysis are not acceptable.

Stability: Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Reference Interval:

 Age 18 years and up                     

    %   absolute number/uL
CD3-CD19+   6-24   77-427
CD20   5-16   60-280

 

Please contact UWHC Flow Cytometry Laboratory at (608) 263-3868 for pediatric reference intervals.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

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