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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: HLA B Locus

Test Code(s): LRBLOCS / HCBLOCS (if Pre-Tx) , LRBLOCB / HCBLOCB (if Post-Tx)

CPT Code(s): 81373

Methodology: Reverse SSO detected by Luminex xMAP (Multi-Analyte Profiling)

Clinical Significance: HLA-B locus low-to-mid level resolution typing typically used to match donor and recipients for organ transplantation and HLA-matched platelet transfusion.  Also may be used to identify the presence of HLA class I alleles associated with some diseases (e.g. B27 for ankylosing spondylitis) For pharmacogenomic relationships (e.g. HLA-B*5701 for Abacavir refer to HLA B for Abacavir Sensitivity [HCHLABAS]. For B*1502 or Carbamazepine sensitivity refer to HLA B for Carbatrol Sensitivity [HCHLABCS]).

Commonly ordered with HLA-A and HLA-C locus for class I HLA typing.

HLA-B27 single antigen testing (for association with Ankylosing Spondylitis) is available separately.  Refer to entry HLA-B27 [XHLA].

Days Performed: Mon-Fri, dayshift by appointment only. Call 263-8815 (option 3) before collection.

Turnaround Time: Stat: Not available stat.
Routine: 5 working days.

Specimen Requirements

Specimen: Blood

Patient Preparation: No transfusions (RBC, WBC, Platelets) within 24 hours prior to collection.

Collection Container: Preferred: Lavender top
Also Acceptable: Lavender top, Yellow top(with ACD-A/B anticoagulant)

Collection Volume: Preferred: 4 mL
Pediatrics: 2 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 4 mL
Pediatrics: 2 mL

Specimen Processing: Do not centrifuge.

Specimen Transport: Transport whole blood specimen at room temperature or refrigerated to UWHC Core Laboratory (B4/220) immediately.

Stability: Ambient: 7 days
Refrigerated: 3 weeks
Frozen: Not acceptable


Test Limitations:

A written interpretive report will be provided.

Additional Information:

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.

HLA Typing, Tissue Typing, B57, Class I, Class 1, Class one, B15, B*1502, B51

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