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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: von Willebrand Disease Type 2B Evaluation

Test Code(s): VW2B / HCVW2B

CPT Code(s): 83519, 85247

Test Description: Test Components: VWD Type 2B Binding von Willebrand Factor Multimers

Testing Site: Testing will be sent to an approved reference laboratory.

Turnaround Time: Routine: 7 - 14 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: Do NOT draw through a line containing heparin.  The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factor and platelets. Tube must be allowed to fill completely.  

Collection Container: Preferred: Light blue top (3.2% NaCitrate)

Collection Volume: Preferred: 3.5 mL
Pediatrics: 2.5 mL

Sample Analyzed: Plasma

Volume Required: Preferred: 1.5 mL
Pediatrics: 1 mL

Specimen Processing: Centrifuge specimen to yield platelet poor plasma (platelet count should be less than 10 K/uL). Separate plasma and transfer to plastic tube or vial as follows. Two aliquots must be submitted.  For adults, one 0.5 mL and one 1.0 mL aliquot. For pediatrics, one 0.2 mL and one 0.7 mL aliquot.
Freeze plasma within 4 hours of collection at -20C or below.

Specimen Transport: Transport whole blood specimen at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen plasma aliquot on dry ice to UWHC Core Laboratory (B4/220).

Stability: Ambient: Not acceptable
Refrigerated: Not acceptable
Frozen: Okay


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