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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: HLA KIR Typing

Test Code(s): KIRB / HCKIRB

CPT Code(s): 81479

Methodology: Reverse SSO detected by Luminex xMAP (Multi-Analyte Profiling)

Clinical Significance: Identifies the presence of KIR (killer cell immunoglobulin-like receptors), some of which may be responsible for binding to HLA ligands and contributing to activation of NK cells. Literature suggests that donor/recipient KIR mismatching may result in lower relapse rate following stem cell transplant.

Days Performed: Scheduled as needed.

Turnaround Time: Stat: Not available stat.
Routine: 3 working days.


Specimen Requirements

Specimen: Blood

Patient Preparation: No transfusions (RBC, WBC, Platelets) within 24 hours prior to collection.

Collection Container: Preferred: Lavender top
Also Acceptable: Yellow top(with ACD-A/B anticoagulant)

Collection Volume: Preferred: 4 mL
Pediatrics: 2 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 4 mL
Pediatrics: 2 mL

Specimen Processing: Do not centrifuge.

Specimen Transport: Do NOT open tube.  Transport whole blood specimen at room temperature to UWHC Core Laboratory (B4/220) immediately.

Stability: Ambient: 5 days
Refrigerated: 5 days
Frozen: Not acceptable


Interpretation

An interpretive report is provided by the laboratory, which will report the presence or absence of each of the 16 known KIR genes. A result of "neutral", "bettter", or "best" will be determined according to the below paper, as calculated by the IPD KIR database KIR-B calculator (www.ebi.ac.uk/ipd/kir/donor_b_content.html)

 

Cooley, DJ Weisdorf, LA Guethlein, JP Klein, T Wang, CT Le, SGE Marsh, D Geraghty, S Spellman, MD Haagenson, M Ladner, E Trachtenberg, P Parham and JS Miller. “Donor selection for natural killer cell receptor genes leads to superior survival after unrelated transplantation for acute myelogenous leukemia”. Blood (2010) 116:2411-9.

Additional Information:

Potential donor DNA is stored in the HLA Laboratory for up to 6 months after HLA typing and may be available for KIR analysis. Please check with the lab to verify availability.

 

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.

KIR killer cell NK

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