| Test Name: |
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JAK2 Mutation V617F Quantitative, PCR
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| Test Code(s): |
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893M50 / HC893M50
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| CPT Code(s): |
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81270
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| Methodology: |
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PCR
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| Clinical Significance: |
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To detect JAK2 mutation V617F associated with myeloproliferative neoplasms. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.
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| Testing Site: |
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Testing will be sent to an approved reference laboratory.
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| Days Performed: |
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Mon-Sat.
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| Turnaround Time: |
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Routine: 3 - 5 days.
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Specimen Requirements
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| Specimen: |
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Bone marrow aspirate
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| Collection Instructions: |
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The bone marrow must be received and processed by WSLH within 24 hours of collection. Bone marrow must be received by the UWHC Core Laboratory Monday-Friday only.
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| Collection Container: |
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Preferred: Lavender top
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| Collection Volume: |
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Preferred: 1 mL
Pediatrics: 0.5 mL
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220) at room temperature (may transport with coolant during hot, >85° weather).
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| Unacceptable Criteria: |
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Bone marrow that is clotted or frozen.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Interpretation provided with results.
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| Test Limitations: |
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Failure to identify a mutant clone by Q-PCR does not rule out the presence of other abnormalities.
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