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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, CAMPTH


CPT Code(s): 88360,86359,88185

Test Description: Test Components: Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3+CD4+ (T-helper/inducer cells), CD3+CD8+ (T-cytotoxic/suppressor cells), CD20 (B-cells), CD4:CD8 ratio

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

Turnaround Time: Routine: 1 - 4 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: An order for a WBC and differential must accompany the request. Outside clients should send a WBC and differential results with the specimen. If no results accompany request, a WBC and differential will be added.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) within 4 hours of collection. Keep specimen at room temperature. DO NOT CENTRIFUGE.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at the time of analysis are not acceptable.

Stability: Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable


Reference Interval:

Age 18 years and up:

   % Absolute number /uL 
 CD3 59-85  767-1903 
 CD3+CD4+ 37-64  481-1464 
 CD3+CD8+ 14-33  247-595 
 CD3-CD20+ 5-16  60-280 
CD4:CD8 1.2-3.7  


Please contact UWHC Flow Cytometry Laboratory at (608)-263-3868 for pediatric reference intervals.

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

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