| Test Name: |
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Flow Cytometry, CAMPTH
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| Test Code(s): |
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CAMPTH / HCCAMPTH
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| CPT Code(s): |
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88360,86359,88185
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| Test Description: |
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Test Components: Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3+CD4+ (T-helper cells), CD3+CD8+ (T-cytotoxic/suppressor cells), CD20 (B-cells), CD4:CD8 ratio
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| Methodology: |
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Flow Cytometry
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| Days Performed: |
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Mon-Fri, dayshift- specimens accepted at anytime.
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| Turnaround Time: |
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Routine: Approximately 2 working days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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An order for a WBC and differential must accompany the request. Outside clients should send a WBC and differential results with the specimen. If no results accompany request, a WBC and differential will be added.
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| Collection Container: |
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Preferred: Lavender top
Also Acceptable:
Lavender top
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Whole Blood
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.5 mL
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220) within 4 hours of collection. Keep specimen at room temperature. DO NOT CENTRIFUGE.
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| Unacceptable Criteria: |
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Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at the time of analysis are not acceptable.
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| Stability: |
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Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Reference Interval:
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Age 18 years and up:
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Absolute number /uL |
| CD3 |
59-85 |
767-1903 |
| CD3+CD4+ |
37-64 |
481-1464 |
| CD3+CD8+ |
14-33 |
247-595 |
| CD3-CD20+ |
5-16 |
60-280 |
| CD4:CD8 |
1.2-3.7 |
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Please contact UWHC Flow Cytometry Laboratory at (608)-263-3868 for pediatric reference intervals.
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| Test Limitations: |
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Absolute values will not be calculated if WBC and differential request or results are not received.
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| Additional Information: |
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This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
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