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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Hepatitis A Ab, Total with Reflex to Hepatitis A Ab, IgM

Test Code(s): HAVRM / HAVHAVM

CPT Code(s): 86708, 86709

Test Description: Test Component:

Hepatitis A Antibody, Total; if reactive, Hepatitis A Antibody, IgM will be performed.


Methodology: Chemiluminescent Immunoassay

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: Not available stat.
Routine: 4 hours.


Specimen Requirements

Specimen: Blood

Patient Preparation: In patients receiving therapy with high doses of biotin (>5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration.

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 4 mL
Pediatrics: 1.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 2 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 12 hours
Refrigerated: 7 days
Frozen: ok; no repeat freeze/thaw cycles


Interpretation

Interpretive Guidelines:

Hepatitis A Antibody, Total:

 

Nonreactive:  Results suggest no past infection. Note: Antibody appears about 4 weeks after infection.

 

Reactive: A positive test for hepatitis A total antibody may indicate past infection, immunization or acute infection. 

 

Hepatitis A Antibody, IgM (if performed):

 

Nonreactive:  A positive test for hepatitis A total antibody in the absence of hepatitis A, IgM may indicate past infection or immunization.  

 

Equivocal:  Recollect if clinically warranted.

 

Reactive:  The presence of IgG and IgM suggests recent or current HAV infection, including recent immunization. 

 

Test Limitations:

Results may be affected if patient has developed heterophillic antibodies due to exposure to animal-based protein products.

 

The assay performance characteristics have not been established for immunocompromised or immunosuppressed patients, newborns, infants or children, cord blood, neonatal specimens, cadaver specimens, heat inactivated specimens and body fluids other than serum.

 

HAA Anti-HAV Hep A

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