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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: FISH- Pediatric ALL Panel

Test Code(s): SLHPED / HCSLHPED

CPT Code(s): 88271 x9, 88275 x6

Test Description: Test Component:

881F67- TEL/AML1 Fusion, t(12;21)(p13;q22), FISH

881F61- BCR/ABL1 Fusion, t(9;22)(q34;q11.2), FISH

870F48- Trisomy 4, FISH, CEP4

870F49- Trisomy 10, FISH, CEP10

870F50- Trisomy 17, FISH, D17Z1

882F84- MLL Rearrangement, FISH

 

Look up individual test components.


Methodology: Fluorescence In Situ Hybridization (FISH)

Clinical Significance: Used for adult and pediatric patients. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Sat.

Turnaround Time: Routine: 3 - 5 days.


Specimen Requirements

Specimen: Blood or bone marrow

Collection Instructions: Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.

Collection Container: Preferred: Green top (with sodium heparin anticoagulant)

Collection Volume: Preferred: 2-3 mL blood or 0.5-1.0 mL bone marrow

Sample Analyzed: Whole Blood or Bone Marrow

Specimen Transport: Transport specimen at room temperature to UWHC Core Laboratory (B4/220). Transport with coolant pack during hot weather (>85F).

Unacceptable Criteria: Bone marrow/blood that is clotted or has been frozen.

Stability: Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Interpretation provided with results.

Test Limitations:

The translocation partner chromosome cannot be determined by this test.

Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen.

 

Additional Information:

If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen.

881F67- TEL/AML1 Fusion, t(12;21)(p13;q22), ETV6/RUNX1; 881F61- BCR/ABL1 Fusion,t(9;22)(q34;q11.2); 870F48- Trisomy 4,CEP4; 870F49- Trisomy 10, CEP10; 870F50- Trisomy 17, D17Z1; 882F84- MLL Gene Rearrangement, 11q23, FISH, acute leukemia

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