UW Hospital and Clinics Lab Test Directory
| Test Name: |
|
FISH- CLL Panel
|
|
|
|
|
|
|
| Test Code(s): |
|
SLHCLL / HCSLHCLL
|
|
|
|
|
|
|
| CPT Code(s): |
|
88271 x5, 88275 x4
|
|
|
|
|
|
|
| Test Description: |
|
Test Component: 870F40- 11q22 deletion/duplication, FISH, ATM gene
870F43- Deletion 13q14, FISH, D13S319
870F47- Deletion 17p13.1, FISH, TP53
870F51- Trisomy 12, FISH, D12Z3
|
|
|
|
|
|
|
| Methodology: |
|
Fluorescence In Situ Hybridization (FISH)
|
|
|
|
|
|
|
| Clinical Significance: |
|
Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.
|
|
|
|
|
|
|
| Testing Site: |
|
Testing will be sent to an approved reference laboratory.
|
|
|
|
|
|
|
| Days Performed: |
|
Mon-Sat.
|
|
|
|
|
|
|
| Turnaround Time: |
|
Routine: 3 - 5 days.
|
|
|
|
Specimen Requirements
|
|
|
|
| Specimen: |
|
Blood or bone marrow
|
|
|
|
|
|
|
| Collection Instructions: |
|
Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.
|
|
|
|
|
|
|
| Collection Container: |
|
Preferred: Green top (with sodium heparin anticoagulant)
|
|
|
|
|
|
|
| Collection Volume: |
|
Preferred: 2-3 mL blood or 0.5-1.0 mL bone marrow
|
|
|
|
|
|
|
| Sample Analyzed: |
|
Whole Blood or Bone Marrow
|
|
|
|
|
|
|
| Specimen Transport: |
|
Transport specimen at room temperature to UWHC Core Laboratory (B4/220). Transport with coolant pack during hot weather (>85°F).
|
|
|
|
|
|
|
| Unacceptable Criteria: |
|
Bone marrow/blood that is clotted or hemolyzed.
|
|
|
|
|
|
|
| Stability: |
|
Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
|
|
|
|
Interpretation
|
|
|
|
|
|
|
Interpretation provided with results.
|
|
|
|
|
|
|
| Test Limitations: |
|
Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen.
|
|
|
|
|
|
|
| Additional Information: |
|
If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen.
|
870F40- 11q22 deletion/duplication, FISH, ATM gene; 870F43- Deletion 13q14, FISH, D13S319; 870F47- Deletion 17p13.1, FISH, TP53; 870F51- Trisomy 12, FISH, D12Z3 chronic lymphocytic
|
