UW Hospital and Clinics Lab Test Directory
| Test Name: |
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FISH- Myeloma Panel
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| Test Code(s): |
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SLHMYL / HCSLHMYL
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| CPT Code(s): |
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88271 x 12, 88275 x 7
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| Test Description: |
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Test Component: 870F40- 11q22 deletion/duplication, FISH, ATM gene
881F62- CCND1/IgH Fusion, t(11;14)(q13;q32), FISH
870F43- Deletion 13q14, FISH, D13S319
870F47- Deletion 17p13.1, FISH, TP53
881F69- FGFR3/IgH Fusion, t(4;14)(p16;q32), FISH
881F70- IGH/MAF Fusion, t(14;16)(q32'q23), FISH
882F82- IGH Rearrangement, FISH
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| Methodology: |
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Fluorescence In Situ Hybridization (FISH)
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| Clinical Significance: |
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Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.
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| Testing Site: |
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Testing will be sent to an approved reference laboratory.
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| Days Performed: |
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Mon-Sat.
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| Turnaround Time: |
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Routine: 3 - 5 days.
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Specimen Requirements
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| Specimen: |
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Blood or bone marrow
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| Collection Instructions: |
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Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.
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| Collection Container: |
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Preferred: Green top (with sodium heparin anticoagulant)
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| Collection Volume: |
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Preferred: 2-3 mL blood or 0.5-1.0 mL bone marrow
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| Sample Analyzed: |
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Whole Blood or Bone Marrow
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| Specimen Transport: |
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Transport specimen at room temperature to UWHC Core Laboratory (B4/220). Transport with coolant pack during hot weather (>85°F).
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| Unacceptable Criteria: |
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Bone marrow/blood that is clotted or has been frozen.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Interpretation provided with results.
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| Test Limitations: |
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Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen.
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| Additional Information: |
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If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen.
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870F40- 11q22 deletion/duplication, ATM gene; 881F62-CCND1/IgH Fusion, 7(11;14)q13;q32); 870F43-D eletion 13q14, D13S319, LAMP1; 870F47-D eletion 17p13.1, TP53, P53, FISH, CLL;881F69- FGFR3/IgH Fusion, t(4:14)(p16:q32),FISH; 881F70- IGH/MAF Fusion, t(14;16)(q32;q23),FISH; 882F82-IGH Rearrangement, FISH
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