| Test Name: |
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FISH- 11q22 deletion/duplication, ATM gene
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| Test Code(s): |
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870F40 / HC870F40
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| CPT Code(s): |
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88271, 88275
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| Methodology: |
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Fluorescence In Situ Hybridization (FISH)
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| Clinical Significance: |
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To detect copy number changes of the ATM gene associated with lymphoid disorders including chronic lymphocytic leukemia (CLL) and multiple myeloma. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.
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| Testing Site: |
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Testing will be sent to an approved reference laboratory.
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| Days Performed: |
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Mon-Sat.
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| Turnaround Time: |
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Routine: 3 - 5 days.
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Specimen Requirements
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| Specimen: |
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Blood or bone marrow
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| Collection Instructions: |
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Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.
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| Collection Container: |
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Preferred: Green top (with sodium heparin anticoagulant)
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| Collection Volume: |
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Preferred: 1-2 mL blood or 0.5-1.0 mL bone marrow
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| Sample Analyzed: |
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Whole Blood or Bone Marrow
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| Specimen Transport: |
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Transport specimen at room temperature to UWHC Core Laboratory (B4/220). Transport with coolant pack during hot weather (>85°F).
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| Unacceptable Criteria: |
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Bone marrow/blood that is clotted or has been frozen.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Interpretation provided with results.
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| Test Limitations: |
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Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen.
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| Additional Information: |
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If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen.
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