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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: ETO/AML1 Fusion Transcript, t(8;21)(q22;q22), PCR

Test Code(s): 887M33 / HC887M33

CPT Code(s): 81401

Methodology: Reverse-Transcriptase PCR

Clinical Significance: Qualitative detection of ETO/AML1 (RUNX1T1/RUNX1) fusion transcripts associated with AML.  Useful in diagnosis of hematologic disorders and for monitoring the disease after treatment or bone marrow transplant.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Fri.

Turnaround Time: Routine: 3 - 5 days.


Specimen Requirements

Specimen: Blood or bone marrow

Collection Instructions: Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 2-3 mL blood or 0.5-1.0 mL bone marrow

Sample Analyzed: Whole Blood or Bone Marrow

Specimen Transport: Transport specimen at room temperature to UWHC Core Laboratory (B4/220). Transport with coolant pack during hot weather (>85F).

Unacceptable Criteria: Sodium heparin is known to inhibit PCR.

Stability: Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Report provides interpretation.

Test Limitations:

Failure to identify an abnormal clone by RT-PCR does not rule out the presence of other abnormalities in the specimen.

cytogenetics
Multiplex RT-PCR Single Assay
RUNX1T1/RUNX1

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