UW Hospital and Clinics Lab Test Directory
| Test Name: |
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E2A/PBX Fusion Transcript, t(1;19)(q23;p13), PCR
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| Test Code(s): |
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887M32 / HC887M32
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| CPT Code(s): |
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81401
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| Methodology: |
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Reverse-Transcriptase PCR
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| Clinical Significance: |
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Qualitative detection of E2A/PBX fusion transcripts associated with ALL. Useful in diagnosis of hematologic disorders and for monitoring the disease after treatment or bone marrow transplant.
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| Testing Site: |
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Testing will be sent to an approved reference laboratory.
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| Days Performed: |
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Mon-Fri.
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| Turnaround Time: |
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Routine: 3 - 5 days.
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Specimen Requirements
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| Specimen: |
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Blood or bone marrow
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| Collection Instructions: |
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Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.
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| Collection Container: |
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Preferred: Lavender top
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| Collection Volume: |
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Preferred: 2-3 mL blood or 0.5-1.0 mL bone marrow
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| Sample Analyzed: |
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Whole Blood or Bone Marrow
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| Specimen Transport: |
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Transport specimen at room temperature to UWHC Core Laboratory (B4/220). Transport with coolant pack during hot weather (>85°F).
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| Unacceptable Criteria: |
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Sodium heparin is known to inhibit PCR.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Report provides interpretation.
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| Test Limitations: |
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Failure to identify an abnormal clone by RT-PCR does not rule out the presence of other abnormalities in the specimen.
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cytogenetics
Multiplex RT-PCR Single Assay
ALL
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