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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: FISH- IgH/MAF Fusion, t(14;16)(q32;q23)

Test Code(s): 881F70 / HC881F70

CPT Code(s): 88271x2, 88275

Methodology: Fluorescence In Situ Hybridization (FISH)

Clinical Significance: To detect the presence of specific fusions of chromosomes associated with myeloma. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Sat.

Turnaround Time: Routine: 3 - 5 days.


Specimen Requirements

Specimen: Blood or bone marrow

Collection Instructions: Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.

Collection Container: Preferred: Green top (with sodium heparin anticoagulant)

Collection Volume: Preferred: 1-2 mL blood or 0.5-1.0 mL bone marrow

Sample Analyzed: Whole Blood or Bone Marrow

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) at room temperature. Transport with coolant pack during hot weather (> 85F).

Unacceptable Criteria: Bone marrow/blood that is clotted or hemolyzed

Stability: Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Interpretation provided with results.

Test Limitations:

Failure to identify an abnormal clone by FISH does not rule out the presence of other abnormalities in the specimen.

Additional Information:

If FISH assay is not performed in conjunction with chromosome analysis there is an additional charge for processing the specimen.

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