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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: BCR/ABL1 Fusion Transcript, t(9:22)(q34;q11.2), Qual, PCR

Test Code(s): 887M31 / HC887M31

CPT Code(s): 81206, 81207

Methodology: Reverse-Transcriptase PCR

Clinical Significance: Detection of BCR/ABL fusion transcripts seen in CML and ALL.  Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Fri.

Turnaround Time: Routine: 3 - 5 days.


Specimen Requirements

Specimen: Blood or bone marrow

Collection Instructions: Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 9 mL blood or 1 mL bone marrow
Pediatrics: 4 mL blood or 1 mL bone marrow

Sample Analyzed: Whole Blood or Bone Marrow

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) at room temperature. Transport with coolant pack during hot weather (> 85F).  

Unacceptable Criteria: Sodium heparin is known to inhibit PCR.

Stability: Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Report provides interpretation.

Test Limitations:

Failure to identify an abnormal clone by RT-PCR does not rule out the presence of other abnormalities in the specimen.

reverse transcription Multiplex RT-PCR single assay cytogenetics

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