UW Hospital and Clinics Lab Test Directory
| Test Name: |
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BCR/ABL1 Fusion Transcript, t(9:22)(q34;q11.2), Qual, PCR
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| Test Code(s): |
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887M31 / HC887M31
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| CPT Code(s): |
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81206, 81207
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| Methodology: |
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Reverse-Transcriptase PCR
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| Clinical Significance: |
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Detection of BCR/ABL fusion transcripts seen in CML and ALL. Aids in diagnosis of hematologic disorders and for monitoring disease after treatment or bone marrow transplant.
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| Testing Site: |
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Testing will be sent to an approved reference laboratory.
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| Days Performed: |
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Mon-Fri.
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| Turnaround Time: |
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Routine: 3 - 5 days.
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Specimen Requirements
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| Specimen: |
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Blood or bone marrow
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| Collection Instructions: |
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Blood or bone marrow must be received and processed by WSLH within 24 hours of collection. Specimen must be received in UWHC Core Laboratory (B4/220) on Monday-Friday only.
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| Collection Container: |
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Preferred: Lavender top
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| Collection Volume: |
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Preferred: 9 mL blood or 1 mL bone marrow
Pediatrics: 4 mL blood or 1 mL bone marrow
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| Sample Analyzed: |
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Whole Blood or Bone Marrow
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220) at room temperature. Transport with coolant pack during hot weather (> 85°F).
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| Unacceptable Criteria: |
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Sodium heparin is known to inhibit PCR.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Report provides interpretation.
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| Test Limitations: |
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Failure to identify an abnormal clone by RT-PCR does not rule out the presence of other abnormalities in the specimen.
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reverse transcription Multiplex RT-PCR single assay cytogenetics
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