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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Human Papilloma (HPV), High Risk with Reflex to Genotyping if Indicated


CPT Code(s): 87624

Test Description: Test Component:


Pap and HPV Testing FAQ's


Methodology: Invader Assay

Clinical Significance: Detection of high risk HPV types

If HPV is requested with a Pap, it must only be ordered within the Pap order as a reflex test. Current ASCCP HPV triage recommendations are based on reflexing HPV off of the Pap result and patient's age. Our lab staff triage for HPV ordering based on your order request within the Pap order and the ASCCP criteria. See the Pap and HPV Testing FAQ's link in the Test Description field above.

An HPV add-on option is available in Health Link for providers wishing to add HPV testing after the Pap has been collected and resulted, within 3 weeks of the collect date.

HPVDNA as normal or office collect status can be ordered when there is insufficient material for HPV testing after the Pap is resulted. Recollect specimen in Thin Prep vial; send to main lab with work station order. Do not reorder the Pap.

Days Performed: Mon-Fri.

Turnaround Time: Routine: 2 - 3 days.

Specimen Requirements

Specimen: See collection instructions.

Collection Instructions: See instructions for Cytology, ThinPrep Pap Test

Collection Container: Preferred: ThinPrep PreservCyt Solution Vial 20 mL

Collection Volume: Preferred: 20 mL

Volume Required: Preferred: 6 mL

Specimen Transport: Place specimen inside a biohazard bag with the request form in the outside pocket of the bag. Transport specimen to UWHC Core Laboratory (B4/220).

Unacceptable Criteria: Failure to tighten the cap on the PreservCyt vial may cause loss of the specimen.

Stability: Ambient: 3 weeks


Report provides interpretation.

Test Limitations:

A Not Detected result does not exclude the possibility of infection with a high risk HPV type.  Virus quantitation is not possible due to the nature of the specimen.

Additional Information:

A positive result (Detected) indicates the presence of DNA from one or more of the following high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68.  This assay does not genotype the virus.  A negative result does not exclude the possibility of infection with a high risk HPV type.  False negative results may be due to low levels of HPV infected cells, inhibitory substances, inappropriate or inadequate sampling, or insufficient cellular material.  Inadequate specimen collection, processing and storage may invalidate test results. 


This test should not be used as the only criterion to form a clinical conclusion; instead, results should be correlated with other test results, patient symptoms and clinical presentation. 


This test is approved by the U.S. Food and Drug Administration.


human papillomavirus high risk

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