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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Respiratory Virus Panel, PCR

Test Code(s): RVPPCR / HCRVPPCR

CPT Code(s): 87633

Methodology: PCR

Clinical Significance: The Respiratory Virus Panel, PCR (RVP) is a qualitative nucleic acid multiplex in vitro diagnostic test for the simultaneous detection and identification of fourteen respiratory virus types and subtypes including: Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza A subtype 2009 H1N1, Influenza B, Respiratory Syncytial Virus (RSV) A, RSV B, Parainfluenza (PIV)1, PIV2, PIV3, Human Metapneumovirus, Human Rhinovirus, Adenovirus species B/E and Adenovirus species C.  The detection and identification of specific viral nucleic acids from individuals exhibiting signs and symptoms of respiratory infection aids in the diagnosis of respiratory viral infection if used in conjunction with other clinical and epidemiological information.

Days Performed: See Additional Information.

Turnaround Time: Routine: 2 - 5 days.


Specimen Requirements

Specimen: Nasopharyngeal Minitip swab (flocked swab), BAL

Collection Instructions: Obtain UTM from UWHC Central Services, item #4010148

Collection Container: Preferred: UTM(Universal Transport Medium)
Also Acceptable: Refrigerated M4 Transport Medium

Collection Volume: Preferred: 2 mL
Pediatrics: 0.5 mL

Volume Required: Preferred: 2 mL
Pediatrics: 0.5 mL

Specimen Transport: Transport specimen to laboratory on coolant pack.

Unacceptable Criteria: Swabs other than nasopharyngeal flocked swabs are not acceptable.

Room temperature M4 (M4RT) is not acceptable.

Stability: Ambient: 2 hours
Refrigerated: 3 days
Frozen: Not acceptable


Interpretation

Expected Results:

Negative

Test Limitations:

Proper collection and transport of specimen is of critical importance in direct detection.

 

Due to the genetic similarity between human rhinovirus and poliovirus, the eSensor RVP cannot reliably differentiate them.  If a polio infection is suspected, a positive eSensor RVP human rhinovirus result should be confirmed using an alternate method (e.g., cell culture).

Additional Information:

Refer to Nasopharyngeal Swab Collection for Respiratory Virus Testing for proper collection.

 

During off-season, test is performed 2-3 times per week.  During peak respiratory virus season, testing frequency will increase as needed.

 

Do not order simultaneously with Influenza A and B, and RSV by PCR [HCFLURSV] or RSV Detection [HCRSVPCR].

 

This test is most appropriate for transplant and immunocompromised patients.  All others should consider Influenza A and B, and RSV by PCR [HCFLURSV].

 

Repeat testing should not be used for test of cure.  If test of cure is needed, please contact the Microbiology Laboratory Director.

 

A negative eSensor RVP result does not exclude the possibility of viral or bacterial infection.  Negative results should not be used as the sole basis for diagnosis, treatment or other patient management decisions.

 

This test was performed using a modification of an FDA approved method.  The performance characteristics of this test were determined by the UWHC Clinical Laboratories and meet the standards for Clinical testing.

RVP

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