UW Hospital and Clinics Lab Test Directory
| Test Name: |
|
Culture, Body Fluid, with Gram Stain
|
|
|
|
|
|
|
| Test Code(s): |
|
BFC/HCBFC, WABF/HCWABF
|
|
|
|
|
|
|
| CPT Code(s): |
|
87070, 87015, 87205, 87075; Additional CPT codes may be added for processing, identification, and susceptibility testing.
|
|
|
|
|
|
|
| Test Description: |
|
Test Component: Culture and Gram stain
|
|
|
|
|
|
|
| Methodology: |
|
Culture, Microscopy
|
|
|
|
|
|
|
| Days Performed: |
|
Daily.
|
|
|
|
|
|
|
| Turnaround Time: |
|
Routine: Preliminary report: 24 hours. Final report: Negative cultures 5 days. Report on specimens from which pathogens are isolated require a minimum of 48 hours for completion.
|
|
|
|
Specimen Requirements
|
|
|
|
| Specimen: |
|
Body fluid
|
|
|
|
|
|
|
| Collection Instructions: |
|
Sterile body fluids such as peritoneal, pleural, pericardial, and synovial can be sent in blood culture bottles obtained from UWHC Central Services. Aseptically transfer equal amounts of fluid into aerobic and anaerobic bottles. Send separate aliquot of fluid in a sterile container for Gram stain.
Pus or aspirates can be sent in a sterile container.
Breast milk specimens should be refrigerated if not transported immediately to laboratory.
A single specimen is sufficient for performing fungal and aerobic/anaerobic culture.
|
|
|
|
|
|
|
| Collection Container: |
|
Preferred: Sterile screw cap container
Also Acceptable:
Bactec® Blood Culture Bottle
|
|
|
|
|
|
|
| Collection Volume: |
|
Preferred: 8 - 20 mL
|
|
|
|
|
|
|
| Sample Analyzed: |
|
Body fluid aliquot
|
|
|
|
|
|
|
| Volume Required: |
|
Preferred: 8 - 20 mL
|
|
|
|
|
|
|
| Specimen Transport: |
|
Transport to UWHC Microbiology Laboratory (B4/231).
|
|
|
|
|
|
|
| Stability: |
|
Ambient: 2 hours
Refrigerated: Not acceptable except for breast milk specimens
Frozen: Not acceptable
|
|
|
|
Interpretation
|
|
|
|
|
Expected Results:
|
|
No Growth
|
|
|
|
|
|
|
| Critical Calls: |
|
View
|
|
|
|
|
|
|
| Additional Information: |
|
Mild to moderate community acquired intraabdominal infections do not need to be cultured.
Complicated community acquired and severe complicated intraabdominal infections as well as immunocompromised patients should be cultured aerobically only. Anaerobic culture is not needed.
|
wound
aerobe
aerobic
anaerobic
Bactec
|
