Skip to Content
UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: JAK2 Mutation V617F, Quantitative, PCR, Blood

Test Code(s): JAK2MD / HCJAK2MD

CPT Code(s): 81270

Methodology: Real-Time PCR followed by Melting Point Analysis

Days Performed: Once a week.

Turnaround Time: Stat: Not available stat.
Routine: 10 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: For bone marrow specimens, order JAK2 Mutation V617F Quantitative, PCR (HC893M50) performed by the State Lab of Hygene.

Testing requires referral screening.  Referral Screening Flow Sheet may be printed from UConnect or see link in Additional Information. Contact UWHC Test Referral office at (608)262-6388 prior to collection.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 2 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 2 mL
Pediatrics: 1.2 mL

Specimen Processing: Do not centrifuge sample.

Specimen Transport: Transport specimen to laboratory immediately. Refrigerate specimen if not delivered immediately. Do not freeze sample.

Stability: Ambient: 24 hours
Refrigerated: 7 days
Frozen: Not acceptable


Expected Results:

Not detected

Test Limitations:

Analytical measurement range for this test is 2.5 to 99.0% Jak2 V617F allele burden. Detection range is 1.0% allele burden and above.

Additional Information:

For whole blood samples in which the Jak2 V617F allele is detected and within the analytical measurement range (AMR), a quantitative value for the mutant allele burden will be reported. If the Jak2 V617F allele burden is detected (≥1.0%) but below 2.5%, a result of "Detected" will be reported. If the Jak2 V617F allele is not detected or the result is below the detection range of 1.0%, a result of "Not detected" will be reported.


A result of 'Not detected" does not rule out the presence of the Jak2 V617F allele. False negative results may be due to inhibitory substances that may be present in the specimen. Inadequate specimen collection, processing and storage may invalidate test results. This test should not be used as the only criterion to form a clinical conclusion. Instead, results should be correlated with other test results, patient symptoms and clinical presentation.


 The performance characteristics of this test were validated by UWHC Clinical Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test; however, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.


A written interpretive report is provided by the laboratory.


A professional fee is associated with this test.


Referral Screening Flow Sheet

janus kinase 2, jak2

Back to Lab Test Directory Index