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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: CGA- Bone Marrow

Test Code(s): SLH812 / HCSLH812

CPT Code(s): 88237, 88264

Methodology: Cell culture, Giemsa/Trypsin/Leishman (GTL) banding

Clinical Significance: Aid in diagnosis of leukemia or pre-leukemic blood disorder. Aid in follow-up after treatment or bone-marrow transplant.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Sat.

Turnaround Time: Stat: 3 - 4 days.
Routine: 6 - 7 days.

Specimen Requirements

Specimen: Bone marrow aspirate

Collection Instructions: When submitting marrow for diagnostic purposes, including a peripheral blood specimen is recommended as blood cultures can provide vital information even when the WBC is very low, and test quality is optimized when both specimens are submitted together.
Bone marrow must be received and processed by WSLH within 24 hours of collection.  Bone marrow must be received by the Core Laboratory  Monday-Friday or before 0900 on Saturday.

Collection Container: Preferred: Heparinized syringe
Also Acceptable: Green top (with sodium heparin anticoagulant)

Collection Volume: Preferred: 1 - 2 mL
Pediatrics: 0.5 mL

Sample Analyzed: Bone Marrow Aspirate

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) at room temperature (may transport with coolant during hot, >85F weather).

Unacceptable Criteria: Bone marrow that is clotted or frozen.  Bone marrow  collected in EDTA, sodium-citrate or anticoagulants other than sodium heparin.

Stability: Ambient: 24 hours
Refrigerated: 24 hours
Frozen: Not acceptable


Expected Results:

46, XX or 46 XY; Apparently normal karyotype, photograph of a representative karyotype included with report.

Test Limitations:

Quality and number of cells may be limited if specimen is not adequate.

Additional Information:

If additional banding is indicated, will perform C-banding or N-banding. If molecular studies with DNA probes are indicated, there will be a small additional charge. If initial results suggest clonal changes, additional cells will be evaluated.

Chromosome Analysis for Hematologic Disorders, Bone Marrow

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