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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, Acute Leukemia

Test Code(s): ALSUB / HCALSUB

CPT Code(s): 88184, 88185x24

Test Description: Test Component:

NOTE: This test is only orderable on Blood specimens. Use Flow Cytometry, Hemepath Consult test (test code FLOWHM/HCFLOWHM) for all other specimen types.

 

B-Cell Antigens-CD19, CD20, CD22,cytoplasmic CD79a, T/NK Cell Antigens-CD2, CD3, cytoplasmic CD3, CD4, CD7, CD56, Myeloid Cell Antigens- CD11b, CD13, CD14, CD15, CD16, CD33, CD64, CD117, MPO, Other Antigens-CD10, CD34, CD38, CD45, HLA-DR, TdT

Note: The flow cytometry immunophenotyping panel may be modified by the staff hematopathologist based on sample size and review of morphology and initial flow data.


Methodology: Flow Cytometry

Clinical Significance: To evaluate the cell surface or cytoplasmic/nuclear antigens present on leukemia or lymphoma cells to determine the lineage of origin and maturation stage. Evaluation of cell morphology (and other relevant testing) is used for interpretation.

Days Performed: Mon-Fri, dayshift- specimens are accepted at any time.

Turnaround Time: Routine: 2 - 5 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: Outside clients should send WBC and differential results and an unstained slide along with specimen.

Collection Container: Preferred: Lavender top
Also Acceptable: Green top

Collection Volume: Preferred: 4 mL
Pediatrics: 2.5 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 4 mL
Pediatrics: 2.5 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 6 hours of collection. Maintain ambient temperature during transport. If sending WBC and differential results, specimen must be received within 8 hours of collection.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Stability: Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

A hematopathologist's written interpretive report is issued as a separate report.

Additional Information:

A professional fee is associated with this test.

 

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

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