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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, Sezary

Test Code(s): SZP / HCPATHI

CPT Code(s): 88184, 88185x9

Test Description: Test Component:

B-Cell Antigens - CD19, Kappa, Lambda, T/NK Cell Antigens - CD3, CD4, CD5, CD7, CD8, Other Antigens - CD26, CD45

Methodology: Flow Cytometry

Clinical Significance: To evaluate the cell surface or cytoplasmic/nuclear antigens present on leukemia or lymphoma cells to determine the lineage of origin and maturation stage. Evaluation of cell morphology (and other relevant testing) is used for interpretation.

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

Turnaround Time: Routine: 2 - 5 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: Outside clients should send WBC and differential results and an unstained slide along with specimen.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 2.5 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 2 mL
Pediatrics: 2 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. Maintain ambient temperature during transport. If sending WBC and differential results, specimen must be received within 8 hours of collection.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable.  Samples greater than 24 hours old at time of analysis are not acceptable.

Stability: Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable


A hematopathologist's written interpretive report is issued as a separate report.

Additional Information:

A professional fee is associated with this test.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

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