UW Hospital and Clinics Lab Test Directory
| Test Name: |
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STR Engraftment Separated Lineages
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| Test Code(s): |
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STRSEP / HCSTRSEP
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| CPT Code(s): |
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81268x2
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| Test Description: |
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Test Component: STR Engraftment Population 1 (CD3+) and STR Engraftment Population 2 (CD33+). Both tests must be ordered at same time and should NOT be ordered individually.
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| Methodology: |
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PCR
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| Clinical Significance: |
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Typically used to assess proportions of donor- and recipient-derived CD3+ and CD33+ cells post stem cell transplant or in cases of suspected graft-vs-host disease.
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| Days Performed: |
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Wed.
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| Turnaround Time: |
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Routine: 10 days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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Testing requires referral screening. Referral Screening Flow Sheet may be printed from UConnect or see link in Additional Information. Contact UWHC Test Referral office at (608)262-6388 prior to collection.
Specific sub-populations of white blood cells may be submitted for analysis. If separated by an outside lab, please identify the population on the label.
Specimens must be received by the UWHC Molecular Diagnostics Lab no later than noon on Fridays or the day before a holiday in order to be processed in a timely manner. Specimens will not remain stable for more than 24 hours.
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| Collection Container: |
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Preferred: Lavender top
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| Collection Volume: |
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Preferred: 8 mL
Pediatrics: 2 mL
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| Specimen Transport: |
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Transport specimen to laboratory.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Interpretive Guidelines:
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Sensitivity of detection of donor(s) in recipient is assessed for each combination of donor(s)/recipient.
| > 95-98% Donor |
Predominantly Donor |
| < 2-5% Donor |
Predominantly Recipient |
| 2-98%, 5-95% Donor |
Donor/Recipient Chimeric |
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| Additional Information: |
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The specimen collected is processed by the UWHC Molecular Diagnostics Laboratory to extract CD3+ and CD33+ cell lineages. Each lineage is tested separately to quantitate and report independently the percentages of donor-derived cells. Test requires pre-transplant DNA samples on file in the laboratory for recipient and donor.
The performance characteristics of this test were validated by UWHC Clinical Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test; however, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.
Referral Screening Flow Sheet
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sorted chimerisms
STRCD3, STR Engraftment Population 1, CD3+
STRCD33, STR Engraftment Population 2, CD33+
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