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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Culture, Tissue, Quant, Aer/Ana with Gram Stain

Test Code(s): QWANC / HCQWANC

CPT Code(s): 87071, 87073, 87205, 87176; Additional CPT codes may be added for processing, identification, and susceptibility testing.

Test Description: Test Component:

Aerobic/anaerobic quantitative culture and Gram stain.


Methodology: Culture, Microscopy

Clinical Significance: Intended use is for burn patients and patients with necrotizing fasciitis.

Days Performed: Daily.

Turnaround Time: Routine: Preliminary report: 24 hours. Final report: Negative cultures 5 days. Report on specimens from which pathogens are isolated require a minimum of 48 hours for completion.


Specimen Requirements

Specimen: Tissue

Collection Container: Preferred: Anaerobic transport (A.C.T. I) tube
Also Acceptable: Sterile screw cap container

Collection Volume: Preferred: 0.4 g
Pediatrics: 0.2 g

Sample Analyzed: Tissue

Volume Required: Preferred: 0.4 g
Pediatrics: 0.2 g

Specimen Transport: Transport to UWHC Microbiology (B4/231) within 1 hour of collection.

Stability: Ambient: 1 hour
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Expected Results:

No growth at 10 to the 3rd CFU/g

wound

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