| Test Name: |
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Insulin, 2 Hour
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| Test Code(s): |
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2HINSL / XINS2
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| CPT Code(s): |
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83525
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| Methodology: |
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Chemiluminescent Immunoassay
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| Request Form: |
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UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.
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| Days Performed: |
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Daily, dayshift.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: Next working day.
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Specimen Requirements
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| Specimen: |
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Blood
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| Patient Preparation: |
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Patient should have nothing to eat or drink (except water) for 8-10 hours before test.
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| Collection Instructions: |
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The patient ingests a solution containing 75 gm of glucose. A blood specimen is drawn 2 hours post-ingestion.
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| Collection Container: |
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Preferred: Red cap with yellow ring (SST)
Also Acceptable:
Red top
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| Collection Volume: |
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Preferred: 3 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.3 mL
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| Specimen Processing: |
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Centrifuge. If a complete barrier has not formed, transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Stability: |
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Ambient: 8 hours
Refrigerated: 24 hours
Frozen: okay
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Interpretation
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Reference Interval:
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0 days and up: 22-79 uIU/mL
The reference interval is based on a 75 gram glucose challenge.
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