Skip to Content
UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Insulin, 2 Hour

Test Code(s): 2HINSL / XINS2

CPT Code(s): 83525

Methodology: Chemiluminescent Immunoassay

Request Form: UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.

Days Performed: Daily, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: Next working day.


Specimen Requirements

Specimen: Blood

Patient Preparation: Patient should have nothing to eat or drink (except water) for 8-10 hours before test.

Collection Instructions: The patient ingests a solution containing 75 gm of glucose.  A blood specimen is drawn 2 hours post-ingestion.

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 8 hours
Refrigerated: 24 hours
Frozen: okay


Interpretation

Reference Interval:

0 days and up:  22-79 uIU/mL

 

The reference interval is based on a 75 gram glucose challenge.

 

Back to Lab Test Directory Index