| Test Name: |
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Culture, Stool, Vibrio
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| Test Code(s): |
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VIBC / HCVIBC
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| CPT Code(s): |
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87046; Additionial CPT codes may be added for processing, identification, and susceptibility testing.
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| Methodology: |
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Culture
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| Days Performed: |
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Daily, 24 hours.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: Preliminary report: 24 hours. Final report: Negative cultures 48 hours. Reports on specimens from which suspicious colonies are seen may require additional time for completion.
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Specimen Requirements
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| Specimen: |
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Stool
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| Patient Preparation: |
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The patient should be cautioned against the use of antacids, barium, bismuth, antidiarrheal medication or oily laxatives prior to the collection of the specimen.
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| Collection Instructions: |
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Pass stool into a clean, dry bedpan. Do not use toilet paper to collect stool. It may be impregnated with barium salts which may be inhibitory. Transfer stool to a Para-Pak Culture & Sensitivity (C&S) kit (orange cap). Check expiration date on Para-Pak kit prior to transfer. If past expiration, do not use.
If sending fresh stool in a sterile container send minimum of 6 mL of stool.
If sending stool in Para-Pak Culture & Sensitivity (C&S) kit fill to red fill line indicated on vial (~3-4 mL of stool).
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| Collection Container: |
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Preferred: Para-PakŪ Culture & Sensitivity (C & S) kit (orange cap)
Also Acceptable:
Cairy-Blair transport media,
Sterile screw cap container
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| Collection Volume: |
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Preferred: See collection instructions.
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| Sample Analyzed: |
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Stool
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| Specimen Transport: |
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Transport to UWHC Microbiology (B4/231) within 2 hours of collection (unpreserved). Specimens collected in Para-Pak Culture and Sensitivity (C&S) kit (orange cap) or Cary-Blair transport media should be transported within 4 days.
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| Unacceptable Criteria: |
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Specimens on patients hospitalized more than 3 days are not acceptable. LIMIT: One specimen every 24 hours.
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| Stability: |
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Ambient: Unpreserved:2 hours; Para-Pak/Cary-Blair: up to 4 days
Refrigerated: Para-Pak/Cary Blair: up to 4 days
Frozen: Not acceptable
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Interpretation
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Expected Results:
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Negative for Vibrio
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| Additional Information: |
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Because these agents do not cause hospital acquired infections, specimens on patients who have been hospitalized greater than 3 days will require approval of the Director of Microbiology or Pathology Resident.
Positive tests are called to a health care provider.
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