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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Culture, Stool, Vibrio

Test Code(s): VIBC / HCVIBC

CPT Code(s): 87046; Additionial CPT codes may be added for processing, identification, and susceptibility testing.

Methodology: Culture

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: Not available stat.
Routine: Preliminary report: 24 hours. Final report: Negative cultures 48 hours. Reports on specimens from which suspicious colonies are seen may require additional time for completion.


Specimen Requirements

Specimen: Stool

Patient Preparation: The patient should be cautioned against the use of antacids, barium, bismuth, antidiarrheal medication or oily laxatives prior to the collection of the specimen.

Collection Instructions: Pass stool into a clean, dry bedpan. Do not use toilet paper to collect stool. It may be impregnated with barium salts which may be inhibitory. Transfer stool to a  Para-Pak Culture & Sensitivity (C&S) kit (orange cap). Check expiration date on Para-Pak kit prior to transfer. If past expiration, do not use.

If sending fresh stool in a sterile container send minimum of 6 mL of stool.

If sending stool in Para-Pak Culture & Sensitivity (C&S) kit fill to red fill line indicated on vial (~3-4 mL of stool).

Collection Container: Preferred: Para-PakŪ Culture & Sensitivity (C & S) kit (orange cap)
Also Acceptable: Cairy-Blair transport media, Sterile screw cap container

Collection Volume: Preferred: See collection instructions.

Sample Analyzed: Stool

Specimen Transport: Transport to UWHC Microbiology (B4/231) within 2 hours of collection (unpreserved). Specimens collected in Para-Pak Culture and Sensitivity (C&S) kit (orange cap) or Cary-Blair transport media should be transported within 4 days.

Unacceptable Criteria: Specimens on patients hospitalized more than 3 days are not acceptable. LIMIT: One specimen every 24 hours.

Stability: Ambient: Unpreserved:2 hours; Para-Pak/Cary-Blair: up to 4 days
Refrigerated: Para-Pak/Cary Blair: up to 4 days
Frozen: Not acceptable


Interpretation

Expected Results:

Negative for Vibrio

Additional Information:

Requires history of recent exposure to salt water or raw seafood.

 

Because these agents do not cause hospital acquired infections, specimens on patients who have been hospitalized greater than 3 days will require approval of the Director of Microbiology or Pathology Resident.

 

Also order Culture, Stool and Shiga toxin for other stool pathogens.

 

Positive tests are called to a health care provider.

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