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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: CA 27.29

Test Code(s): CA2729 / X2729

CPT Code(s): 86300

Methodology: Chemiluminescent Immunoassay

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Routine: Next working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.15 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 8 hours
Refrigerated: 48 hours
Frozen: okay


Reference Interval:

0-39 U/mL

Test Limitations:

The Siemens ADVIA Centaur method is used.  Results obtained with different assay methods or kits cannot be used interchangeably.

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.

Additional Information:

The CA 27.29 assay is intended for use as an aid in monitoring patients previously treated for Stage II or Stage III breast cancer. Serial testing in patients who are clinically free of disease should be used in conjunction with other clinical methods used for the early detection of cancer recurrence. This test is also intended for use as an aid in the management of breast cancer patients with metastatic disease by monitoring the progression or regression of disease in response to treatment.


Patients with confirmed breast carcinoma frequently have levels of CA 27.29 within the range observed in healthy individuals. Additionally, elevated levels of CA 27.29 can be observed in patients with nonmalignant diseases.


cancer antigen 2729

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