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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: ACTH

Test Code(s): ACTH / XACTH

CPT Code(s): 82024

Methodology: Chemiluminescent Immunoassay

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: 1 working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 2 mL
Pediatrics: 0.6 mL

Sample Analyzed: Plasma

Volume Required: Preferred: 1 mL
Pediatrics: 0.25 mL

Specimen Processing: Centrifuge.  Separate plasma from cells and freeze immediately.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport frozen if coming from outreach location.

Stability: Ambient: 5 hours
Refrigerated: 5 hours
Frozen: 30 days


Reference Interval:

Age 0 days and up:  7 - 45 pg/mL


Reference interval established for 8-10 AM collection. No established reference interval for PM collection.

Test Limitations:

Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with in vitro immunoassays. Samples from patients routinely exposed to animals or animal serum products can demonstrate this type of interference potentially causing an anomalous result.

Adrenocorticotropic Hormone

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