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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Q Fever Ab


CPT Code(s): 86638 x2

Test Description: Test Component:

Quantitative total antibody IFA testing with phase 1 and phase 2 antigens.

Methodology: Indirect Fluorescent Antibody

Clinical Significance: Serodiagnosis of Coxiella burnetii infection.

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Once a week.

Turnaround Time: Routine: 1 - 7 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: Acute and convalescent sera recommended for diagnostic testing.

Collection Container: Preferred: Red top

Collection Volume: Preferred: 4 mL
Pediatrics: 3 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge. Transfer cell free serum to plastic vial. Refrigerate. Store at 2-8C.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.  


Interpretive Guidelines:

Diagnostically significant titer of 1:256 or greater indicates current or recent infection with this Rickettsial agent.

Test Limitations:

This test will only provide evidence of Coxiella burnetii exposure.  Additional tests may be needed to rule out other causes of illness.

Additional Information:

Paired sera are necessary for a definitive serological confirmation of a Q fever infection.

Coxiella burnetii Antibodies


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