| Test Name: |
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AFB, Sputum
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| Test Code(s): |
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AFB / HCAFB
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| CPT Code(s): |
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87015, 87206, 87116
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| Test Description: |
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Test Component: Acid fast stain and culture with identification for mycobacteria. Species identification may include HPLC, DNA probes, biochemical testing, growth characteristics and gene sequencing. Mycobacterial susceptibility available based on species isolated.
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| Methodology: |
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Microscopy and Culture
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| Testing Site: |
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Testing will be sent to an approved reference laboratory.
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| Days Performed: |
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Mon-Fri.
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| Turnaround Time: |
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Routine: Smear/Stain: 1 - 3 days. Final report: 1 - 8 weeks.
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Specimen Requirements
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| Specimen: |
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Sputum
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| Patient Preparation: |
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Collect specimen(s) prior to chemotherapy.
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| Collection Instructions: |
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Follow packaging instructions included with State Lab Acid Fast Bacillus (AFB) collection kit (CS Item# 2200232). Collect a series of 3 specimens on DIFFERENT days over a 3-5 day period.
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| Collection Container: |
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Preferred: State Lab Acid Fast Bacillus (AFB) collection kit
Also Acceptable:
Sterile screw cap container
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| Sample Analyzed: |
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Sputum
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| Volume Required: |
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Preferred: 5 - 10 mL
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220).
Transport to laboratory as soon as possible. Refrigerate if specimen submission is delayed.
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| Unacceptable Criteria: |
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Saliva or 24-hour pooled sputums are not acceptable specimens.
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Interpretation
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| Additional Information: |
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Culture results include: Negative; Species identification when mycobacterial growth is detected; Culture overgrown with non acid-fast organisms.
Mycobacterial susceptibility testing recommended for M. tuberculosis isolates.
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