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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Biotinidase


CPT Code(s): 82261

Methodology: Spectrophotometry

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Mon-Fri.

Turnaround Time: Routine: 3 - 7 days.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top

Collection Volume: Preferred: 3 mL
Pediatrics: 1.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.6 mL

Specimen Processing: Spin immediately. Critical frozen.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) immediately.  Specimen must arrive in the Core Lab within 1 hour of collection.

Outreach Specimen Transport: Transport frozen serum to UWHC Core Laboratory (B4/220) on dry ice.

Unacceptable Criteria: Exposure to heat or multiple freeze-thaw cycles.

Stability: Ambient: 1 hour
Refrigerated: 1 hour
Frozen: 1 month


Report provides interpretation. Contact WSLH Biochemical Genetics Lab at (608) 262-6386 for further information.

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