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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Brucella Ab

Test Code(s): SLI2 / XBRUC

CPT Code(s): 86622

Test Description: Test Components: Test will detect antibody to Brucella abortus, Brucella melitensis and Brucella suis.

Methodology: Direct Agglutination

Testing Site: Testing will be sent to an approved reference laboratory.

Days Performed: Once a week.

Turnaround Time: Routine: 2 - 6 days.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top

Collection Volume: Preferred: 4 mL
Pediatrics: 1.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge. Transfer cell free serum to plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.


Interpretive Guidelines:

A positive titer of 1:80 or greater on a single serum indicates current infection. Low titers may indicate a previous exposure to a related organism and may not be diagnostically significant in individuals with repeated exposure to brucellae.

Test Limitations:

Cross-reactions may occur between Brucella and Francisella species.

Additional Information:

Brucellosis is often related to occupational exposure.  Serological results must be critically assessed along with clinical findings and occupational factors before a diagnosis is made.

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