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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, PNH

Test Code(s): FPNH / HCPNH

CPT Code(s): 88184, 88185x7

Test Description: Test Component:

Granulocytes that are identified based on CD45 vs Side Scatter (SS) and  CD15-positivity are analyzed for GPI anchored proteins, FLAER and CD24. Monocytes that are indentified based on CD45 vs SS and CD64 -positivity are analyzed for GPI anchored proteins FLAER and CD14. Red cells expressing CD235a are analyzed for GPI anchored protein CD59.

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

Turnaround Time: Stat: Not available stat.
Routine: 2 - 5 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: An order for a CBC and differential must accompany request. Outside clients should send CBC and differential results along with specimen. If no results accompany request, a CBC and differential will be added (see transport and limitations for more information).

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. Maintain ambient temperature during transport.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Stability: Ambient: 6 hours if CBC and Differential results do not accompany specimen. 24 hours if CBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable


A hematopathologist's written interpretive report is issued as a separate report.

Test Limitations:

This test is run by comparing the patient's sample to a "normal control" sample.

Additional Information:

 A professional fee is associated with this test.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

PNH Testing Flow Cytometry - PNH Analysis FLAER based and CD59
Paroxysmal Nocturnal Hemoglobinuria

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